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P-9005-48?EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
P-9005-96 EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
? The EpiQuik? m6A RNA Methylation Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify N6-methyladenosine (m6A or 6mA) in RNA. It is suitable for a direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses. The kit has the following advantages and features:
Colorimetric ELISA-like assay using an m6A antibody with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes.
? High sensitivity, of which the detection limit can be as low as 10 pg of m6A.
Unique binding solution allows RNA >70 nts to be tightly bound to the wells, which enables quantification of m6A from both mRNA and ncRNA such as tRNA, rRNA, and snRNA.
? Optimized antibody and enhancer solutions allow high specificity to m6A, with no cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA.
? Universal positive and negative controls are included, which are suitable for quantifying m6A from any species.
Strip-well microplate format makes the assay flexible for manual or high throughput analysis.
Simple, reliable, and consistent assay conditions.
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